REGENERATION AND TRANSPLANTATIONNetrin G-2 ligand mRNA is downregulated in spinal motoneurons after sciatic nerve lesionBerg, Alexander; Zelano, Johan; Cullheim, StaffanAuthor Information Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden Correspondence to Alexander Berg, Department of Neuroscience, Karolinska Institutet, Retzius väg 8, Stockholm SE-171 77, Sweden Tel: +46 8 524 878 51; fax: +46 8 32 09 88; e-mail: [email protected] Received 6 April 2010 accepted 27 May 2010 NeuroReport: August 4, 2010 - Volume 21 - Issue 11 - p 782-785 doi: 10.1097/WNR.0b013e32833cadd8 Buy Metrics Abstract Netrin G-2 ligand (NGL-2) and synaptic adhesion like molecules induce synapses in vitro. We investigated the expression of these molecules in a model of CNS synaptic detachment and restoration in vivo. After axotomy of spinal motoneurons, synapses are lost from the somata of lesioned motoneurons. We could not detect any synaptic adhesion like molecule mRNA in the spinal cord, but signal for NGL-2 mRNA was seen in motoneurons. The signal for NGL-2 decreased after sciatic nerve transection and sciatic nerve crush. After regeneration, the levels of NGL-2 mRNA were partially restored after sciatic nerve transection, but completely restored after sciatic nerve crush. We conclude that axotomized motoneurons decrease their NGL-2 expression and that the restoration of NGL-2 expression mirrors the restoration of synaptic inputs. © 2010 Lippincott Williams & Wilkins, Inc.