CHEMICAL, SENSESNMDA receptor in intestinal enteroendocrine cell, STC-1Fukunaga, Yoichiro; Kimura, Mayuko; Saitoh, Osamu Author Information Department of Bio-Science, Faculty of Bio-Science, Nagahama Institute of Bio-Science and Technology, Nagahama-shi, Shiga, Japan Correspondence to Professor Osamu Saitoh, PhD, Department of Bio-Science, Faculty of Bio-Science, Nagahama Institute of Bio-Science and Technology, 1266 Tamura-cho, Nagahama-shi, Shiga 526-0829, Japan Tel/fax: +81 749 64 8165; e-mail: [email protected] Received 24 April 2010 accepted 11 May 2010 Supplemental digital content is available for this article. Direct URL citations appear in the printed text and are provided in the HTML and PDF versions of this article on the journal's Website (www.neuroreport.com). NeuroReport: August 4, 2010 - Volume 21 - Issue 11 - p 772-776 doi: 10.1097/WNR.0b013e32833bfd17 Buy SDC Metrics Abstract We earlier showed that intestinal STC-1 cells can respond to five basic taste stimuli. Here, we investigated which receptors or channels are involved in the sensing of umami in STC-1 cells. T1R family and metabotropic glutamate receptors were not involved. In contrast, we found that N-Methyl-D-aspartate (NMDA) receptors are expressed in STC-1 cells by RT-PCR analysis. Using a calcium-imaging technique, we also observed that a specific agonist NMDA induces the increase in the intracellular Ca2+ concentration in STC-1 cells. We also found that pretreatment with NMDA enhances sweet-induced response in STC-1 cells, suggesting important roles of L-Glu-signaling in the chemosensory system of the gastrointestinal tracts. © 2010 Lippincott Williams & Wilkins, Inc.