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D609 inhibits the proliferation of neural progenitor cells

Kalluri, Haviryaji S.G.a; Dempsey, Robert J.a b

doi: 10.1097/WNR.0b013e32833b46f6
Cellular, Molecular and Developmental Neuroscience

We examined the effect of tricyclodecan-9-yl-xanthogenate (D609), a phosphatidylcholine-specific phospholipase C inhibitor, on the proliferation of adult neural progenitor cells in vitro. D609 (100 μM) decreased the proliferation of neural progenitor cells as measured by the proliferation assay, bromodeoxyuridine incorporation, and cell counting without causing cell death. Immunoblot analysis showed a decrease in the expression of cyclin D1 and phosphorylation of retinoblastoma after the incubation of cells with D609. These results suggest that D609 inhibits the proliferation of neural progenitor cells by reducing the cyclin D1 content resulting in decreased phosphorylation of retinoblastoma and subsequent inhibition of cell division.

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aDepartment of Neurological Surgery

bCardiovascular Research Center, University of Wisconsin, Madison, Wisconsin, USA

Correspondence to Haviryaji S.G. Kalluri, PhD, Department of Neurological Surgery, CSC F4/313, 600 Highland Avenue, University of Wisconsin, Madison, WI 53792, USA

Tel: +1 608 265 5443; fax: +1 608 263 1728; e-mail:

Received 24 March 2010 accepted 21 April 2010

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© 2010 Lippincott Williams & Wilkins, Inc.