MOLECULAR NEUROSCIENCERole of collapsin response mediator protein-2 in neurite outgrowth of PC12 cellsBork, Kaya; Karbe, Yvonne; Pollscheit, Juliane; Glaubitz, Nicole; Nöhring, Sabine; Horstkorte, RüdigerAuthor Information Institute of Physiological Chemistry, Martin-Luther University of Halle-Wittenberg, Halle/Saale, Germany Correspondence to Dr Rüdiger Horstkorte, PhD, Martin-Luther-University Halle-Wittenberg, Hollystr. 1, Halle/Saale 06114, Germany Tel: +49 345 5573873; fax: +49 345 5573804; e-mail: firstname.lastname@example.org Kaya Bork, Yvonne Karbe, and Juliane Pollscheit contributed equally to this study Received 19 February 2010 accepted 31 March 2010 NeuroReport: June 23rd, 2010 - Volume 21 - Issue 9 - p 641-645 doi: 10.1097/WNR.0b013e32833a7d53 Buy SDC Metrics Abstract Collapsin response mediator proteins (CRMPs) are involved in cell differentiation and axonal growth. In this study, we investigated neurite outgrowth and the expression of CRMP-2 in PC12 cells. Nondifferentiated PC12 cells hardly express CRMP-2, but the expression of CRMP-2 increases with neurite outgrowth. We established a stable CRMP-2-overexpressing PC12 cell line and found that PC12 cells, which constitutively overexpress CRMP-2, were unable to extend neurites after stimulation with the nerve growth factor or the neural cell adhesion molecule, a procedure that promotes neurite outgrowth in untransfected cells. In contrast, a PC12 cell line deficient in CRMP-2 extends neurites after the stimulation of nerve growth factor or neural cell adhesion molecule. © 2010 Lippincott Williams & Wilkins, Inc.