CELLULAR, MOLECULAR AND DEVELOPMENTAL NEUROSCIENCERegulation of katanin-P60 levels by LECT2 adjusts microtubular morphologyKoshimizu, Yohei; Ohtomi, MichikoAuthor Information Department of Biomolecular Science, Graduate School of Science, Toho University, Miyama, Funabashi, Chiba, Japan Correspondence to Dr Yohei Koshimizu, Department of Biomolecular Science, Graduate School of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan Tel/fax: +81 29 861 2716; e-mail: firstname.lastname@example.org Received 27 February 2010 accepted 31 March 2010 NeuroReport: June 23rd, 2010 - Volume 21 - Issue 9 - p 646-650 doi: 10.1097/WNR.0b013e32833a7d6f Buy SDC Metrics Abstract Leukocyte cell-derived chemotaxin 2 (LECT2) facilitates neuritic extension in cultured hippocampal neurons during initial development. However, the functions of LECT2 in neuritic extension are poorly understood. To elucidate these functions, we examined microtubular morphology and levels of katanin-P60, a microtubule-severing enzyme, in cultured hippocampal neurons from wild-type mice and LECT2 knockout (KO) mice. Microtubules in LECT2-KO mice exhibited fragmentation and were shorter than those of wild-type mice. Furthermore, the expression of katanin-P60 in LECT2-KO mice was significantly elevated when compared with wild-type mice at 1 day in vitro (DIV1) and 4. Our results suggest that LECT2 regulates neuritic extension through microtubular morphallaxis through the control of katanin-P60 levels. © 2010 Lippincott Williams & Wilkins, Inc.