CELLULAR, MOLECULAR AND DEVELOPMENTALGeneration of neuronal cells from human peripheral blood mononuclear cellsHorschitz, Sandra; Meyer-Lindenberg, Andreas; Schloss, Patrick Author Information Department of Psychiatry and Psychotherapy, Central Institute of Mental Health, University of Heidelberg/Medical Faculty Mannheim, Mannheim, Germany Correspondence to Dr Patrick Schloss, Biochemical Laboratory, Central Institute of Mental Health, J5, Mannheim 68159, Germany Tel: +49 621 1703 2901; fax: +49 621 1703 6255; e-mail: [email protected] Received 13 October 2009 accepted 28 October 2009 NeuroReport: February 17, 2010 - Volume 21 - Issue 3 - p 185-190 doi: 10.1097/WNR.0b013e328334be4e Buy Metrics Abstract We have examined the potency of two methods for the neuronal differentiation of embryonic stem cells on the generation of neuronal cells from human blood cells. A mixture of mononuclear cells from peripheral blood cells expressing monocytic, hematopoietic, and mesenchymal cell surface markers were exposed to all-trans retinoic acid, epidermal growth factor, and basic fibroblast growth factor (method A), or epidermal growth factor, fibroblast growth factor 8b, sonic hedgehog and ascorbic acid (method B). Both methods led to the generation of neuronal cells as judged by changes in morphology and the expression of the neuronal markers microtubule-associated protein type 2, tau, and β-tubulin III. Differentiation according to method B favoured the development of neurons also expressing the dopamine transporter. © 2010 Lippincott Williams & Wilkins, Inc.