The purpose of this study was to investigate the developmental change of epithelial Na+ channel function in epithelial cells of rat Reissner's membrane. The Reissner's membrane was isolated from the upper turn of the cochlea at different stages of development. The vibrating probe technique was chosen to measure transepithelial currents under short circuit conditions. Amiloride-sensitive Na+ current in the Reissner's membrane developed postnatally between days 3 and 5, which was completely blocked by amiloride. This tendency remained from days 5 through 14. Development of Na+ transport in epithelial cells of the Reissner's membrane may contribute considerably to the accomplishment of low Na+ concentration in the endolymph during early neonatal period.
Department of Otorhinolaryngology, Seoul National University College of Medicine, Seoul National University Hospital and Sensory Organ Research Institute, Seoul National University Medical Research Center, Seoul, Korea
Correspondence to Dr Jun Ho Lee, MD, PhD, Department of Otorhinolaryngology, Seoul National University College of Medicine, Seoul National University Hospital, 28 Yeongon-dong, Chongro-gu, Seoul 110-744, Korea
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The results in this study were reported at the midwinter meeting of the Association for Research in Otolaryngology in 2008.
Received 22 June 2009 accepted 24 June 2009