BEHAVIORAL, INTEGRATIVE AND CLINICAL NEUROSCIENCEHeat loss during exercise is related to serotonin activity in the preoptic areaRodrigues, Alex G.a c; Soares, Danusa D.b; Marubayashi, Umekoa; Coimbra, Cândido CelsoaAuthor Information aDepartment of Physiology and Biophysics, Institute of Biological Science bDepartment of Physical Education, School of Physical Education, Physical Therapy and Occupational Therapy, Federal University of Minas Gerais cInstitute of Biological and Health Sciences, Pontifícia Universidade Católica, MG, Belo Horizonte, Minas Gerais, Brazil Correspondence to Dr Cândido Celso Coimbra, PhD, Department of Physiology and Biophysics, Institute of Biological Science, Federal University of Minas Gerais, Av. Antonio Carlos, 6627, 31270-901, Belo Horizonte, Minas Gerais, Brazil Tel: +55 31 3409 2936; fax: +55 31 3409 2924; e-mail: [email protected] Received 19 February 2009 accepted 12 March 2009 NeuroReport: May 27th, 2009 - Volume 20 - Issue 8 - p 804-808 doi: 10.1097/WNR.0b013e32832b8c90 Buy Metrics Abstract To investigate the influence of the central cholinergic system on thermoregulation and brain serotonin concentration during exercise; 2 μl of physostigmine (5×10−3 M) or saline solution was injected into the lateral cerebral ventricle of running rats. At fatigue, brains were quickly removed and serotonin and 5-hydroxyindoleacetic acid were measured in the preoptic area, hypothalamus, frontal cortex, and hippocampus. Physostigmine injection attenuated hyperthermia and exercise-induced heat storage that was closely related to the serotonin content in the preoptic area. Physostigmine treatment also increased the heat dissipation by decreasing core temperature threshold for vasodilation. In conclusion, our data indicated that stimulation of the central cholinergic system promotes heat dissipation in running rats that is related to decreased serotonin content in the preoptic area. © 2009 Lippincott Williams & Wilkins, Inc.