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A high-resolution large area serotonin map of a live rat brain section

Kaushalya, Sanjeev K.; Nag, Suman; Ghosh, Himanish; Arumugam, Senthil; Maiti, Sudipta

doi: 10.1097/WNR.0b013e3282fd6946
Brain Imaging

We employ three-photon microscopy to produce a high-resolution map of serotonin autofluorescence in a rat midbrain section (covering more than half of the brain), to quantitatively characterize serotonin distribution and release in different areas of a live brain slice. The map consists of a tiling of ∼160 contiguous optical images (covering an area of ∼27 mm2 with sub-μm resolution in 20 min), and is recorded before and after inducing depolarization. We observe that the total serotonin exocytosed from the somata in the raphe is quantitatively comparable with regions containing a high density of serotonergic processes. Our results demonstrate that high-resolution, wide-area, dynamic neurotransmitter mapping is now possible.

Department of Chemical Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai, India

Correspondence to Dr Sudipta Maiti, D-304, Biophotonics Lab, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai, 400005, India

Tel: +9122 2278 2716; fax: +9122 2280 4610; e-mail: maiti@tifr.res.in

Received 22 January 2008; accepted 15 February 2008

© 2008 Lippincott Williams & Wilkins, Inc.