MOLECULAR NEUROSCIENCERit contributes to neurite outgrowth triggered by the alpha subunit of GoKim, Seung Hyuna; Kim, Soyeonb; Ghil, Sung Hoa Author Information aDepartment of Life Science, Kyonggi University bDepartment of Anatomy, School of Medicine, Ajou University, Suwon, South Korea Correspondence to Dr Sung Ho Ghil, PhD, Department of Life Science, Kyonggi University, Suwon 443-760, South Korea Tel: +82 31 249 9649; fax: +82 31 249 9649; e-mail: [email protected] Received 10 January 2008; accepted 24 January 2008 NeuroReport: March 26, 2008 - Volume 19 - Issue 5 - p 521-525 doi: 10.1097/WNR.0b013e3282f9e473 Buy Metrics Abstract Heterotrimeric GTP-binding protein transduce signals initiated by a variety of hormones and neurotransmitters. Go, a member of the Go/Gi family, is the most abundant heterotrimeric GTP-binding protein in nervous tissues and has been implicated in neuronal differentiation. The mechanism by which Go modulates neuronal differentiation has not been, however, fully elucidated. Here, we identified small GTPase Rit as an interacting partner of the α-subunit of Go (Goα). The biochemical characterizations of Goα::Rit interaction revealed that Rit is a candidate downstream effector for Goα. Furthermore, dominant negative Rit inhibited Goα-induced neurite outgrowth and Erk phosphorylation in Neuro2a cells. These results suggest that Rit may be involved in the signaling pathway for Goα-mediated neuronal differentiation. © 2008 Lippincott Williams & Wilkins, Inc.