MOLECULAR NEUROSCIENCESubthalamic stimulation increases striatal tyrosine hydroxylase phosphorylationReese, Renea b *; Winter, Christinec d *; Nadjar, Agnesa; Harnack, Danielc; Morgenstern, Rudolfe; Kupsch, Andreasc; Bezard, Erwana; Meissner, Wassiliosa f Author Information aCNRS UMR 5227, Bordeaux, France bDepartment of Neurology, Christian-Albrechts-University Kiel, Germany cDepartment of Neurology, Charité Campus Virchow dDepartment of Psychiatry and Psychotherapy eInstitute of Pharmacology and Toxicology, Charité Campus Mitte, University Medicine Berlin, Germany fDepartment of Neurology, CHU de Bordeaux, Hôpital du Haut-Lévêque, Bordeaux, France Correspondence to Dr Wassilios Meissner, CNRS UMR 5227, Université Victor Segalen, 146 rue Léo Saignat, 33076 Bordeaux Cedex, France Tel: +33 557 571 687; fax: +33 556 901 421; e-mail: [email protected] *Rene Reese and Christine Winter contributed equally to the writing of this article. Received 4 October 2007; accepted 4 November 2007 NeuroReport: January 22, 2008 - Volume 19 - Issue 2 - p 179-182 doi: 10.1097/WNR.0b013e3282f417b4 Buy Metrics Abstract Subthalamic stimulation enhances striatal tyrosine hydroxylase activity, which is regulated by phosphorylation at different serine residues. Western blotting was performed to investigate phosphorylation at the serine residues 19, 31 and 40 in striatal tissue of rats that had received subthalamic stimulation or sham stimulation for 2 h. In animals that were killed directly after stimulation, the tyrosine hydroxylase protein content was unchanged, whereas phosphorylation at the serine residue 19 was increased and phosphorylation at the serine residues 31 and 40 tended to be higher compared with controls. By contrast, tyrosine hydroxylase protein content and phosphorylation were similar in rats that were killed 24 h after stimulation. Our results suggest that subthalamic stimulation may increase tyrosine hydroxylase activity via increased phosphorylation. © 2008 Lippincott Williams & Wilkins, Inc.