NEUROENDOCRINOLOGYIn-vitro and in-vivo antagonistic action of an anti-amylin SpiegelmerBilik, Kerstin U.a; Ergüven, Elifa; Klussmann, Svenb; Jarosch, Florianb; Wielinga, Peter Y.a; Lutz, Thomas A.a; Riediger, Thomasa Author Information aInstitute of Veterinary Physiology, University of Zurich, Zurich, Switzerland bNOXXON Pharma AG, Berlin, Germany Correspondence to PD Dr Thomas Riediger, Institute of Veterinary Physiology, University of Zurich, Winterthurerstrasse 260, 8057 Zurich, Switzerland Tel: +41 44 635 8815; fax: +41 44 635 8932; e-mail: [email protected] Received 13 August 2007; accepted 30 August 2007 NeuroReport: November 19, 2007 - Volume 18 - Issue 17 - p 1855-1859 doi: 10.1097/WNR.0b013e3282f1ab04 Buy Metrics Abstract The anorectic and dipsogenic effects of the pancreatic hormone amylin are mediated by the area postrema and the subfornical organ. We tested the effectiveness of a new amylin antagonist, a so-called RNA Spiegelmer, by electrophysiological in-vitro recordings from the rat subfornical organ and by immunohistological c-Fos studies in the area postrema. Amylin's excitatory effect on subfornical organ neurons was blocked by the anti-amylin Spiegelmer. Peripheral administration 5 h prior to amylin also suppressed the amylin-induced activation (c-Fos expression) in the area postrema. The biostable anti-amylin Spiegelmer may be therapeutically beneficial in conditions associated with high plasma amylin levels, such as cancer anorexia occurring during certain pancreatic tumors. © 2007 Lippincott Williams & Wilkins, Inc.