MEMBRANE AND CELLULAR BIOPHYSICS AND BIOCHEMISTRYIntracellular dynamics of calcyon, a neuron-specific vesicular proteinKruusmägi, Markusa; Zelenin, Sergeya; Brismar, Hjalmara b; Scott, LenaaAuthor Information aDepartment of Woman and Child Health, Karolinska Institutet bDepartment of Cell Physics, Royal Institute of Technology, Stockholm, Sweden Correspondence to Lena Scott, Department of Woman and Child Health, Karolinska Institutet, Astrid Lindgrens Children's Hospital Q2:09, S171 76 Stockholm, Sweden Tel: +46 8 517 773 19; fax: +46 8 517 773 28; e-mail: Lena.Scott@ki.se Received 3 July 2007; accepted 11 July 2007 NeuroReport: October 8th, 2007 - Volume 18 - Issue 15 - p 1547-1551 doi: 10.1097/WNR.0b013e3282f03f51 Buy Metrics Abstract Calcyon is a brain-specific protein, implicated in clathrin-mediated endocytosis. In this descriptive study we show that calcyon is exclusively expressed in neurons, and localized in moving vesicles. The movement of calcyon-containing vesicles was dependent on temperature and on intact microtubules, in addition these vesicles were colocalized with a marker for endocytosed plasma membrane proteins, suggesting that calcyon vesicles follow the endocytic recycling pathway. We also show using evanescent wave microscopy that there is a pool of ready releasable calcyon vesiclesaccumulated beneath the plasma membrane. We conclude that the mobility and storage properties of calcyon-containing vesicles imply that they play a role in brain plasticity. © 2007 Lippincott Williams & Wilkins, Inc.