Secondary Logo

Institutional members access full text with Ovid®

Share this article on:

Voltage-clamp errors cause anomalous interaction between independent ion channels

Farris, Hamilton E.CA; Ricci, Anthony J.


In voltage-clamp, uncompensated series resistance results in steady-state voltage errors that scale with the amplitude of the elicited current and are often correctable offline. However, while investigating mechanoelectric transduction currents at hair cells' resting potential, voltage-gated calcium channels and calcium-activated potassium channels (BK) were activated in voltage-clamp by displacing the sensory hair bundle. This resulted from steady-state voltage errors (<1.5 mV) induced by series resistance changing the holding potential. Thus, uncompensated series resistance, interacting with an elicited current, resulted in a voltage error that could induce the erroneous activation of other currents. This error is not correctable offline. Recognizing this type of error is critical when investigating multiple voltage-dependent conductances with steep voltage dependence.

Neuroscience Center and Kresge Hearing Labs, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA

CACorresponding Author:

Received 23 March 2005; accepted 22 April 2005

© 2005 Lippincott Williams & Wilkins, Inc.