Voltage-clamp errors cause anomalous interaction between independent ion channelsFarris, Hamilton E.CA; Ricci, Anthony J.NeuroReport: June 21st, 2005 - Volume 16 - Issue 9 - p 943-947 AUDITORY AND VESTIBULAR SYSTEMS Abstract Author InformationAuthors In voltage-clamp, uncompensated series resistance results in steady-state voltage errors that scale with the amplitude of the elicited current and are often correctable offline. However, while investigating mechanoelectric transduction currents at hair cells' resting potential, voltage-gated calcium channels and calcium-activated potassium channels (BK) were activated in voltage-clamp by displacing the sensory hair bundle. This resulted from steady-state voltage errors (<1.5 mV) induced by series resistance changing the holding potential. Thus, uncompensated series resistance, interacting with an elicited current, resulted in a voltage error that could induce the erroneous activation of other currents. This error is not correctable offline. Recognizing this type of error is critical when investigating multiple voltage-dependent conductances with steep voltage dependence. Neuroscience Center and Kresge Hearing Labs, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA CACorresponding Author: email@example.com Received 23 March 2005; accepted 22 April 2005 © 2005 Lippincott Williams & Wilkins, Inc.