AUDITORY AND VESTIBULAR SYSTEMSInnervation of stem cell-derived neurons into auditory epithelia of miceMatsumoto, Masahiro1; Nakagawa, Takayuki1 CA; Higashi, Toru1; Kim, Tae-Soo1; Kojima, Ken1 2; Kita, Tomoko1 3; Sakamoto, Tatsunori1 4; Ito, Juichi1Author Information 1Department of Otolaryngology-Head and Neck Surgery 2Establishment of International COE for Integration of Transplantation Therapy and Regenerative Medicine 3Horizontal Medical Research Organization Kyoto University, Graduate School of Medicine, Kyoto, Japan 4Organogenesis and Neurogenesis Group, Center for Developmental Biology, RIKEN, Kobe, Japan CACorresponding Author: firstname.lastname@example.org Received 22 February 2005; accepted 14 March 2005 NeuroReport: May 31st, 2005 - Volume 16 - Issue 8 - p 787-790 Buy SDC Abstract This study examined the potential of embryonic stem cell-derived neurons as transplants for cell therapy for the primary loss of spiral ganglion neurons. To assess the ability of embryonic stem cell-derived neurons for innervation into auditory epithelia, they were cocultured with auditory epithelium explants of mice for 7 days. Histological analysis demonstrated massive elongation of neurites from embryonic stem cell-derived neurons toward auditory hair cells. Embryonic stem cell-derived neurites were adjacent to or surrounding hair cells, and exhibited expression of synaptophysin, a marker for synaptic vesicles. These findings demonstrate the ability of embryonic stem cell-derived neurons for reinnervation into auditory epithelia, indicating a high potential of embryonic stem cell-derived neurons as transplants for replacing spiral ganglion neurons. © 2005 Lippincott Williams & Wilkins, Inc.