NEUROPHARMACOLOGY AND NEUROTOXICOLOGYLack of ATP-evoked GABA and glutamate release in the hippocampus of P2X7 receptor−/− micePapp, Lilla; Vizi, E. Sylvester; Sperlágh, BeátaCAAuthor Information Laboratory of Molecular Pharmacology, Institute of Experimental Medicine, Hungarian Academy of Sciences, H-1450 Budapest, POB 67, Hungary CACorresponding Author: email@example.com Received 21 July 2004; accepted 31 August 2004 NeuroReport: October 25th, 2004 - Volume 15 - Issue 15 - p 2387-2391 Buy SDC Abstract In this study we revealed the participation of P2X7 receptors in the modulation of electrical stimulation and ATP-evoked GABA and glutamate release from mouse hippocampal slices. Whereas the uptake of radioactivity was not changed, the electrical stimulation-induced release of both [3H]glutamate and [3H]GABA was decreased in the hippocampus of P2X7 receptor-deficient mice. ATP (10 mM) elicited [3H]glutamate and [3H]GABA efflux in wild-type mice, which was inhibited by the non-selective P2 receptor antagonist pyridoxal-phosphate-6-azophenyl-2′,4′-disulphonic acid tetrasodium (30 μM) and the P2X7-selective antagonist Brilliant Blue G (1 μM). The ATP-evoked release of both GABA and glutamate was virtually absent in the knockout mice. These results indicate that ATP facilitates GABA and glutamate release in the hippocampus by a mechanism involving P2X7R. © 2004 Lippincott Williams & Wilkins, Inc.