GLIAL CELLSEffects of hyperbaric oxygen on GDNF expression and apoptosis in spinal cord injuryYu, YiminCA; Matsuyama, Yukihiro; Yanase, Makoto1; Ito, Sachiko2; Adachi, Kayo2; Satake, Kotaro; Ishiguro, Noki; Kiuchi, Kazutoshi3 Author Information Department of Orthopaedic Surgery, Nagoya University School of Medicine, 466-8500 Nagoya 1Nagoya Kyoritsu Hospital, 454-0933 Nagoya 2National Institute for Longevity Sciences, 474-8552 Aichi 3Department of Biomolecular Science, Faculty of Engineering, Gifu University, 501-1193 Gifu, Japan CACorresponding Author: [email protected] Received 20 May 2004; accepted 5 August 2004 NeuroReport: October 25, 2004 - Volume 15 - Issue 15 - p 2369-2373 Buy Abstract The effects of hyperbaric oxygen treatment on the progress of secondary damage following traumatic spinal cord injury were investigated. The early onset of hyperbaric oxygen treatment significantly diminished the number of apoptotic cells 1 day after the injury. However, hyperbaric oxygen did not influence the proliferation of macrophages or activated microglia. The gene expression of glial cell line-derived neurotrophic factor (GDNF) and inducible nitric oxide synthetase (iNOS) was significantly attenuated 1 day after the injury in the hyperbaric oxygen groups compared with the control group. The down-regulation was confirmed by immunohistochemical staining. Early hyperbaric oxygen treatment was shown to effectively suppress the progress of apoptosis perhaps via the inhibition of iNOS gene despite the down-regulation of the GDNF gene. © 2004 Lippincott Williams & Wilkins, Inc.