Comparative effects of high glucose on different adult sympathetic neurons in culture

Abstract

In vivo, diabetes causes neuropathy in the sympathetic celiac/superior mesenteric ganglion (CG/SMG) but not the superior cervical ganglion (SCG). Therefore, our aim was to compare the effects of high glucose in vitro on adult rat SCG and CG/SMG neurons. High glucose decreased viability and neurite outgrowth and increased TUNEL staining in freshly dissociated neurons from both ganglia. In cultures established for 24 h before exposure to high glucose, a significant (p<0.01) decrease in neurite-bearing neurons from CG/SMG but not SCG occurred. CG/SMG neurons took longer (p<0.05) to initiate neurite outgrowth and had lower (p<0.002) anti-oxidant defence enzyme activity. These properties may contribute to the selective development of neuropathy in CG/SMG in diabetes in vivo.