MOTOR SYSTEMSChanges in purine levels and adenosine receptors in kindled seizures in the ratÅdén, UlrikaCA; O'Connor, William T.1; Berman, Robert F.2Author Information Department of Woman and Child Health, Karolinska Institutet, S-171 76 Stockholm, Sweden 1Department of Human Anatomy and Physiology, The Conway Institute of Biomedical and Biomolecular Research, The National Neuroscience Network, University College Dublin, National University of Ireland, Dublin, Ireland 2Department of Neurological Surgery, University of California at Davis, Davis, CA, USA CACorresponding Author: firstname.lastname@example.org Received 27 March 2004; accepted 11 May 2004 NeuroReport: July 19th, 2004 - Volume 15 - Issue 10 - p 1585-1589 doi: 10.1097/01.wnr.0000133227.94662.c9 Buy SDC Metrics Abstract Adenosine is an inhibitory modulator of neuronal activity and its possible involvement in seizures is of interest. We have examined changes in adenosine, its metabolites and receptors in brains of hipppocampus-kindled rats, a model of partial epilepsy. Purine levels were measured by in vivo microdialysis and showed a small increase in adenosine and a dramatic increase in its metabolites after kindled seizures. Adenosine A1 receptor binding using [3H]DPCPX was unaltered after seizures, whereas A1 agonist stimulated binding of GTP[γ-35S] and A1 mRNA expression increased in the CA3 and other regions. Striatal adenosine A2A mRNA and receptor binding with [3H]SCH-58261 decreased. These findings indicate that kindled seizures increase adenosine release and metabolism and induces adaptive changes in adenosine receptors. © 2004 Lippincott Williams & Wilkins, Inc.