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3D MRI of the in vivo vestibulo-cochlea labyrinth during Gd-DTPA-BMA uptake

Allen Counter, S.1 2 CA; Zou, Jing2; Bjelke, Börje2 3; Klason, Tomas4

Auditory and Vestibular Systems

The morphology, time-course and volume of the in vivo uptake of the T1 contrast agent gadolinium (Gd) in the perilymphatic vestibulo-cochlea labyrinth, including the utricle, saccule, semicircular canals and scalae of the guinea pig inner ear were analyzed as Fourier transform signal intensity enhancement levels by 3D MRI at 4.7 T. The uptake of Gd as a function of time in the perilymphatic space of the vestibular labyrinth was shown by ANOVA and PLSD post hoc tests to be significantly less (p < 0.05) than that of the scala tympani of the cochlea 10, 30, 60 and 90 min after i.v. injection. Experimentally induced fistulae resulted in MRI detected morphological and quantitative alterations in Gd concentration in the perilymphatic labyrinthine space. The findings demonstrate that Gd-enhanced 3D MRI of the perilymphatic space may be used to examine the morphology, kinetics and intravenous substance delivery in the in vivo mammalian vestibulo-cochlea labyrinth.

1Neurology Department, Harvard Medical School, Massachusetts General Hospital, Harvard University Biological Laboratories, 16 Divinity Avenue, Cambridge, MA 02138, USA

2Department of Clinical Neuroscience, Karolinska Institutet, MR Centrum, Karolinska Hospital, S-17176 Stockholm

3KTH and KI Nano and Microtechnology Center, Department of Radiology, Center for Surgical Sciences, Huddinge Hospital SE-141 86 Stockholm

4Konsultfirman tekn. Dr. Tomas Klason Sturevägen 4B, S-133 38 Saltsjöbaden, Sweden

CACorresponding Author:

Received 13 May 2003; accepted 8 July 2003

© 2003 Lippincott Williams & Wilkins, Inc.