NEUROCHEMISTRYMatrix metalloproteinase expression in the olfactory epitheliumTsukatani, Toshiaki1 4; Fillmore, Helen L.2 3; Hamilton, Heather R.2; Holbrook, Eric H.1; Costanzo, Richard M.1 CA Author Information 1Departments of Physiology 2Surgery, PO Box 980551, Virginia Commonwealth University, Medical College of Virginia Campus, Richmond, VA 23298-0551 3Research Service, Hunter Holmes McGuire Department of Veterans Affairs Medical Center, Richmond, VA, USA 4Department of Otorhinolaryngology, School of Medicine, Kanazawa University, 13-1 Takaramachi Kanazawa, 920-0934, Japan Received 2 October 2002; accepted 25 February 2003 CACorresponding Author: [email protected] NeuroReport: June 11, 2003 - Volume 14 - Issue 8 - p 1135-1140 Buy Abstract The olfactory epithelium contains neuronal progenitor cells capable of continuous neurogenesis and is a unique model for studying neural degeneration, regeneration, axon outgrowth and recovery from injury. Matrix metalloproteinases (MMPs), and tissue inhibitors of metalloproteinases (TIMPs), have been implicated in cell turnover, development, migration, and metastatic processes. We used Western blot and immunohistochemistry to determine whether MMP-2 and associated proteins TIMP-2 and membrane type 1 matrix metalloproteinase (MT1-MMP) are present in the olfactory epithelium of mice. We found MMP-2 expression localized to the olfactory basal cells and immature neurons. After injury-induced neural degeneration, MMP-2 and MT1-MMP levels decreased while TIMP-2 levels increased. However, following 35 days of neurogenesis and cell replacement TIMP-2 and MT1-MMP returned to control levels. The results show a correlation between MMP and TIMP levels and the stages of neural degeneration, regeneration and recovery of the olfactory epithelium following injury. © 2003 Lippincott Williams & Wilkins, Inc.