NEUROCHEMISTRYIn situ Ca2+ imaging of odor responses in a coronal olfactory epithelium sliceOmura, Masayo; Sekine, Hisaki1; Shimizu, Takao1; Kataoka, Hiroshi; Touhara, KazushigeCA Author Information Department of Integrated Biosciences, Rm 201, Graduate School of Frontier Sciences, University of Tokyo, Chiba 277-8562 1Department of Biochemistry and Molecular Biology, Faculty of Medicine, The University of Tokyo, Japan and CREST of Japan Science and Technology Corporation, Tokyo 113-0033, Japan Received 1 January 2003; accepted 29 January 2003 CACorresponding Author: [email protected] NeuroReport: June 11, 2003 - Volume 14 - Issue 8 - p 1123-1127 Buy SDC Erratum Abstract An in situ Ca2+-imaging technique was adopted to monitor odorant responses of more than several hundreds of neurons simultaneously in an intact coronal slice of the mouse olfactory epithelium. The sensitivity and resolution of the slice Ca2+-imaging were high enough to distinguish between olfactory receptor neurons with threshold concentrations in a one-order difference for a particular odorant at the single-cell level. Increasing odorant concentrations resulted in increases in the numbers of odorant-responsive neurons, which were visualized in situ in the coronal slice. The methodology established in this study is a powerful tool to visualize spatial distributions of odorant responsive neurons at a cellular resolution, and to construct odor maps in a coronal view of the olfactory epithelium. Erratum The Publisher would like to apologise to Kazushige Touhara, the corresponding author of the article referenced below , for an error in the presentation of his e-mail address. The address should read: [email protected] NeuroReport. 14(12):1653, August 26, 2003. © 2003 Lippincott Williams & Wilkins, Inc.