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Basic fibroblast growth factor induces TGF-β release in an isoform and glioma-specific manner

Dhandapani, Krishnan M.; Wade, Marlene F.; Mahesh, Virendra B.; Brann, Darrell W. CA

Neurophysiology, Basic And Clinical

The aim of the current study was to determine whether basic fibroblast growth factor (bFGF), regulates the release of transforming growth factor-β1 (TGF-β1) from C6 glioma cells. The results of the study show that bFGF (2, 5 and 10 ng/ml) dose dependently induced the release of TGF-β1 from C6 glioma cells, with the 10 ng/ml dose inducing a 2- to 3-fold increase of TGF-β1 levels. This effect was evident as early as 6 h following treatment, with maximal levels observed at 18 h. The effect of bFGF was largely on latent TGF-β1, and was isoform specific, as bFGF had no effect on TGF-β2 release. The bFGF effect on TGF-β1 was also glioma specific, as no such stimulatory effect was observed in rat cortical astrocytes.

Institute of Molecular Medicine and Genetics, Neurobiology Program, and Department of Neurology, 1120 15th Street, Medical College of Georgia, Augusta, GA 30912, USA

CACorresponding Author

Received 18 October 2001 accepted 7 December 2001

© 2002 Lippincott Williams & Wilkins, Inc.