AUDITORY AND VESTIBULAR SYSTEMSTransfection of young guinea pig vestibular cells in vitro with an adenovirus vectorSuzuki, MitsuyaCA; Yamasoba, Tatsuya1; Kondo, Kenji1; Kaga, Kimitaka1Author Information Department of Otolaryngology, Tokyo Metropolitan Police Hospital, 2-10-41, Fujimi, Chiyoda-ku, Tokyo 102-0071; 1Department of Otolaryngology, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan CACorresponding Author Received 16 August 2001; accepted 15 October 2001 Neuroreport: December 21st, 2001 - Volume 12 - Issue 18 - p 4013-4017 Buy SDC Abstract This study shows distributions of lacZ-positive cells in the vestibular labyrinthine explants of young guinea pigs with mature ears. When adenovirus lacZ vectors were administered to the vestibular labyrinth following removal of the otoconial membrane, lacZ-positive cells were observed in the mesothelial cells in the perilymphatic space, in the sensory and supporting cells in the utricle and ampulla, and in the transitional and dark cells in the ampulla. When the otoconial membrane was preserved, lacZ-positive cells were not distributed in the utricular sensory epithelium. These findings suggest that adenovirus vectors can transform a variety of vestibular epithelial cells, but that it is difficult for adenovirus vectors to pass through the otoconial membrane. © 2001 Lippincott Williams & Wilkins, Inc.