VISION, RETINASodium nitroprusside selectively induces apoptotic cell death in the outer retina of the ratJu, Won-Kyu; Chung, Ik-Whan; Kim, Keun-Young; Gwon, Jae-Sung; Lee, Mun-Yong; Oh, Su-Ja; Chun, Myung-HoonCAAuthor Information Department of Anatomy, College of Medicine, The Catholic University of Korea, 505 Banpo-dong, Socho-gu, Seoul 137-701, Korea CACorresponding Author Received 29 August 2001; accepted 24 October 2001 Neuroreport: December 21st, 2001 - Volume 12 - Issue 18 - p 4075-4079 Buy SDC Abstract Sodium nitroprusside (SNP), an NO donor, was studied for its effects on apoptosis in rat retinal neurons. TUNEL-positive cells were observed in the outer nuclear layer (ONL), but not in the inner retina after SNP treatment. Inner retinal neurons died by necrosis. No photoreceptor cells were found in the ONL after seven days. Immunoblotting confirmed that neur- nal NO synthase expression increased up to 5 days (∼170% of control levels), and then declined by 7 days, suggesting that NO induces apoptosis in the ONL, and that inner retinal neurons die by necrosis due to glutamate from damaged photoreceptors. © 2001 Lippincott Williams & Wilkins, Inc.