MEMBRANE AND CELLULAR BIOPHYSICS AND BIOCHEMISTRYManganese stimulates stellation of cultured rat cortical astrocytesLiao, Su-Lan; Chen, Chun-JungCAAuthor Information Department of Education and Research, Taichung Veterans General Hospital, No. 160, Sec. 3, Taichung-Gang Road, Taichung 40705, Taiwan, Republic of China CACorresponding Author Received 8 August 2001; accepted 3 October 2001 Neuroreport: December 21st, 2001 - Volume 12 - Issue 18 - p 3877-3881 Buy SDC Abstract We investigated the effect of manganese on the morphology of cultured rat cortical astrocytes. Astrocytes exhibited flattened, polygonal morphology in the absence of stimulation, and differentiated into process-bearing stellate cells following exposure to MnCl2. MnCl2-induced stellation was a reversible process, which depended on the presence of extracellular free manganese. MnCl2-induced stellation did not stop with the introduction of pertussis toxin, PD98059, SB203580, phorbol 12-myristat 13-acetate, SQ22536, or LY83583. Alternatively, MnCl2-induced stellation did stop when exposed to colchicine and sodium orthovanadate, suggesting the involvement of the cytoskeletal elements and orthovanadate-sensitive protein tyrosine phosphatase. MnCl2 might function as a factor regulating astrocyte morphology. © 2001 Lippincott Williams & Wilkins, Inc.