Genetics Of Nervous System DiseaseDifferentially expressed genes in rat dorsal root ganglia following peripheral nerve injuryKim, Dong-Sun; Lee, Sang-Ji; Park, So-Yun; Yoo, Hea-Jin; Kim, Shin-Hee1; Kim, Kwang-Jin1; Cho, Hee-JungCAAuthor Information Department of Anatomy, School of Medicine, Kyungpook National University, 2-101, Dongin Dong, Taegu, 700-422; 1Department of Physiology, School of Medicine, Chungnam National University, Moonwha-1 Dong, Taejon, Korea CACorresponding Author Received 9 July 2001; accepted 15 August 2001 NeuroReport: October 29th, 2001 - Volume 12 - Issue 15 - p 3401-3405 Buy Abstract Ordered differential display PCR was used to identify differentially expressed genes in rat dorsal root ganglia at 7 days following chronic constriction injury (CCI) of the sciatic nerve. Fourteen differentially displayed cDNA bands were isolated, cloned and verified by RT-PCR. The four mRNAs were increased, which included mRNAs encoding heat shock protein 27, fatty acid binding protein, apolipoprotein D and one novel gene. Six down-regulated clones were microtubule-associated protein 1B, protein tyrosine phosphatase α, Kv1.2 channel, myelin protein SR13, medium-sized neurofilament protein, and one novel gene. Our results show that many differentially regulated genes after CCI may play a role in nerve degeneration and/or regeneration and provide a molecular framework for understanding the peripheral mechanism underlying neuropathic pain. © 2001 Lippincott Williams & Wilkins, Inc.