NeurochemistryProfile of phosphoprotein labelling in organotypic slice cultures of rat hippocampusTavares, Alexandre; Cimarosti, Helena; Valentim, Lauren; Salbego, ChristianneCAAuthor Information Dep. de Bioquímica-ICBS, UFRGS, Av. Ramiro Barcelos, 2600, Anexo I, Porto Alegre RS, Brasil CACorresponding Author Received 10 May 2001; accepted 27 June 2001 Neuroreport: August 28th, 2001 - Volume 12 - Issue 12 - p 2705-2709 Buy Abstract In recent years organotypic slice cultures of hippocampal tissue of rats have been widely used to study factors involved in neuronal death. Here we used 2D electrophoresis to study the phosphoprotein profile in such cultures and the effect of oxygen/glucose deprivation on this profile. Cultures were prepared from 7-day-old rats. After 14 days in culture the phosphorylation profile in the cultures, as shown by phospho- protein markers undergoing developmental change, closely resembled the profile of fresh tissue from 23-day-old rats. The results suggest that this model could be a good method to observe the development of the tissue and its response to an ischaemic lesion © 2001 Lippincott Williams & Wilkins, Inc.