Molecular NeuroscienceExpression of the high-affinity glutamate transporter EAAT4 in mammalian cerebral cortexMassie, Ann; Vandesande, Frans; Arckens, LutgardeCAAuthor Information Laboratory of Neuroendocrinology and Immunological Biotechnology, Katholieke Universiteit Leuven, Naamsestraat 59, B-3000 Leuven, Belgium CACorresponding Author Received 19 October 2000; accepted 23 November 2000 Neuroreport: February 12, 2001 - Volume 12 - Issue 2 - p 393-397 Buy Abstract RT-PCR, immunocytochemistry and Western blotting were used to study the expression of the glutamate transporter EAAT4 in the cerebral cortex of cat and mouse. By means of RT-PCR we were able to detect EAAT4 mRNA in the cerebral cortex of both species. Sequencing ensured the specificity of the amplified fragment. Immunocytochemistry and Western blotting enabled us to localize EAAT4 protein in cat and mouse cerebral cortex. Intense EAAT4 immunoreactivity was found in the soma and dendrites of neurons mainly of layers II, III and V. For both species, the signal in the cerebellum was very intense and confined to the molecular and Purkinje cell layer. © 2001 Lippincott Williams & Wilkins, Inc.