MOLECULAR NEUROSCIENCENeurons and ECM regulate occludin localization in brain endothelial cellsSavettieri, Giovanni1; Liegro, Italia Di2; Catania, Caterina2; Licata, Luana2; Pitarresi, Giovanna Laura2; D'Agostino, Stefania2; Schiera, Gabriella2; De Caro, Viviana3; Giandalia, Giulia3; Giannola, Libero Italo3; Cestelli, Alessandro2,4Author Information 1Istituto di Neuropsichiatria, Policlinico Universitario Paolo Giaccone, Palermo 2Dipartimento di Biologia Cellulare e dello Sviluppo Alberto Monroy, Viale delle Scienze, Parco d'Orleans, Universit àdegli Studi, 90128 Palermo 3Dipartimento di Chimica e Tecnologie Farmaceutiche, Palermo, Italy 4Corresponding Author: Alessandro Cestelli Acknowledgments: This work was supported both by the Italian Ministero della Ricerca Scientifica e Tecnologica, and by the University of Palermo. Receieved 12 January 2000; accepted 27 January 2000 NeuroReport: April 7, 2000 - Volume 11 - Issue 5 - p 1081-1084 Buy Abstract We report that extracellular matrix and neurons modulate the expression of occludin, one of the main components of tight junctions, by rat brain endothelial cells (RBE4.B). Of the three extracellular matrix proteins which we tested (collagen I, collagen IV, and laminin), collagen IV stimulated at the best the expression of occludin mRNA. The corresponding protein, however, was not synthesized. Significant amounts of occludin accumulated only when RBE4.B cells were cultured on collagen IV-coated inserts, in the presence of cortical neurons, plated on laminin-coated companion wells. Finally, occludin segregated at the cell periphery, only when endothelial cells were co-cultured with neurons for ≥ 1 week. © 2000 Lippincott Williams & Wilkins, Inc.