MOLECULAR NEUROSCIENCEQuantitative PCR analysis of AMPA receptor composition in two paradigms of global ischemiaAlsbo, Carsten W.1,2; Wrang, Maria Louise1; Johansen, Flemming Fryd1; Diemer, Nils Henrik1Author Information 1Laboratory of Neuropathology, Institute of Molecular Pathology, University of Copenhagen, 11, Frederik V's vej, DK-2100 Copenhagen, Denmark 2Corresponding Author: Carsten W. Alsbo Received 6 October 1999; accepted 14 November 1999 NeuroReport: February 7, 2000 - Volume 11 - Issue 2 - p 311-315 Buy Abstract Quantitative PCR was used to analyse the expression of GluR1, GluR2, GluR2 flip, GluR2 flop and GluR3 mRNA in animals after ischemia and tolerance-inducing ischemia. The ischemic animals showed a decrease in the GluRs to ∼30%, except for GluR2-flip, which decreased to 75%. The tolerance animals displayed regulation of GluR1 to 75%, GluR2 and GluR2-flop to 283% and 265% respectively. We did not find a correlation between GluR2 regulation and cell loss in the ischemic group. The selective upregulation of GluR2/GluR2 flop in tolerant animals indicates a possible mechanism for enhanced AMPA receptor desensitisation leading to tolerance to ischemia. © 2000 Lippincott Williams & Wilkins, Inc.