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Immunohistochemical localization of CDK5 activator p39 in the rat brain

Honjyo, Yasuyuki; Kawamoto, Yasuhiro; Nakamura, Shinichi; Nakano, Satoshi; Akiguchi, Ichiro

Molecular Neuroscience

WE examined the immunohistochemical localization of p39, the regulatory subunit of cyclin-dependent kinase 5 (Cdk5), in the adult rat brain. Cdk5 requires either p39 or p35 to assume its active form in the mammalian CNS. We developed a specific antibody against p39, and localized p39-immunoreactivity in the rat brain. p39-immunoreactivity was observed in the neuronal somata of the cerebral cortex, hippocampus and basal ganglia, and also in the Purkinje cells. Immunopositive astrocytes and oligodendrocytes were scattered thourghout the rat brain. p35 immunoactivity has been observed exclusively in neurons and was never detected in glial cells. The present results suggest that p39 and p35 may have different functional roles in the regulation of Cdk5 in the rat CNS.

1Department of Neurology, Faculty of Medicine, Kyoto University, Kyoto, 606-8507, Japan

2Corresponding Author: Shinichi Nakamura

ACKNOWLEDGEMENTS: This work was supported in part by a Grant-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Science, Sports and Culture of Japan and by research grants (C-07670712 and C-09670654) from the Ministry of Education, Science, Sports and Culture of Japan.

Received 18 August 1999; accepted 7 September 1999

© 1999 Wolters Kluwer Health | Lippincott Williams & Wilkins