Regeneration and TransplantationRegeneration of transected spinal cord in young adult rats using freeze-dried alginate gelSuzuki, Kyoko1; Suzuki, Yoshihisa1,5; Ohnishi, Katsunori2; Endo, Katsuaki3; Tanihara, Masao4; Nishimura, Yoshihiko1 Author Information 1Department of Plastic and Reconstructive Surgery, Section of Surgery for Sensory and Motor System, Division of Surgery, Kyoto University Graduate School of Medicine, Sakyo-ku, Kyoto 606-8507, Japan 2Department of Neurology, Kyoto University Graduate School of Medicine, Sakyo-ku, Kyoto 606-8507, Japan 3Department of Physiology, Kyoto University Graduate School of Medicine, Sakyo-ku, Kyoto 606-8507 Japan 4Graduate School of Material Science, Nara Institute of Science and Technology, Nara, 630-0101, Japan 5Corresponding Author: Yoshihisa Suzuki ACKNOWLEDGEMENTS: This study was supported by Grants-in-Aid 11307037 and 11877240 for scientific research of the Japanese Ministry of Science, Education and Culture, and by a grant from the Japanese Society for Promotion of Science (JSPSRFTF 96100203). Received 13 July 1999; accepted 26 July 1999 NeuroReport: September 29, 1999 - Volume 10 - Issue 14 - p 2891-2894 Buy Abstract WE have recently reported that freeze-dried alginate gel, which was developed in our laboratory, enhanced peripheral nerve regeneration. The purpose of this study was to examine whether alginate gel is capable of promoting nerve regeneration in the severed spinal cord of adult mammals. Using Wistar rats at 30 days of age (P30), the T9–T10 spinal cord was totally resected and alginate gel was implanted across the gap. Forty-five days after surgery myelinated and unmyelinated axons regenerated throughout the gap with remaining alginate gel. The elongated axons established electrophysiologically functional projections across the gap. In conclusion, freeze-dried alginate gel could be a promising material as an artificial nerve guide for repair of injured central nervous system. © 1999 Lippincott Williams & Wilkins, Inc.