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AMPA-type glutamate receptor subunits are expressed in the avian cochlear hair cells and ganglion cells

Reng, Diana1; Hack, Iris2; Müller, Marcus1; Th Smolders, Jean W.1,3

Auditory and Vestibular Systems

THE cellular localization of AMPA-type glutamate receptor subunits was examined in the pigeon inner ear using subunit specific polyclonal antibodies (GluR1–4). In the auditory ganglion cell bodies immunoreactivity for the subunits GluR2/3 and GluR4, but not for GluR1 was detected. The hair cells showed diffuse immunoreactivity for GluR4. Additionally, immunostaining for the subunits GluR2/3 and GluR4 was present below the hair cells. These results indicate that the AMPA type glutamate receptors play a role in neurotransmission at the hair cell afferent synapse in the avian auditory system.

1Klinikum der J. W. Goethe Universität, Physiologisches Institut III, Theodor-Stern-Kai 7, 60590 Frankfurt/Main, Germany

2Max-Planck-Institut für Hirnforschung, Deutschordenstr. 46, 60528 Frankfurt/Main, Germany

3Corresponding Author: Jean W. Th Smolders

ACKNOWLEDGEMENTS: We would especially like to thank Ulrike Zimmermann and PD Dr Marlies Knipper for the help on the Western blot and immunoprecipitation. This work was supported by the Deutsche Forschungsgemeinschaft (SFB 269, B1 and B4) and the Graduiertenkolleg Neuronale Plastizität

Received 28 April 1999; accepted 5 May 1999

© 1999 Lippincott Williams & Wilkins, Inc.