Autonomic Nervous SystemInjection of antisense oligos to nNOS into nucleus tractus solitarii increases blood pressureMaeda, Masanobu1,4; Hirano, Hideyasu2; Kudo, Hideaki3; Doi, Yoshiaki3; Higashi, Ken2; Fujimoto, Sunao3Author Information 1Department of Systems Physiology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health (UOEH), 1-1, Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan 2Department of Biochemistry, School of Medicine, University of Occupational and Environmental Health (UOEH), 1-1, Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan 3Department of Anatomy, School of Medicine, University of Occupational and Environmental Health (UOEH), 1-1, Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan. 4Corresponding Author: Masanobu Maeda ACKNOWLEDGEMENTS. This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan. (10670074) and in part Kimura Memorial Heart Foundation/Pfizer Pharmaceuticals Inc. Grant for Research on Autonomic Nervous System and Hypertension. Received 21 April 1999; accepted 30 April 1999 NeuroReport: June 23rd, 1999 - Volume 10 - Issue 9 - p 1957-1960 Buy Abstract WE investigated the cardiovascular effects of bilateral microinjection of antisense oligodeoxynucleotides (oligos) into the nucleus tractus solitarii (NTS) to neuronal nitric oxide synthase (nNOS) to suppress the expression of nNOS molecular biologically. In urethane-anesthetized, paralyzed Wistar-Kyoto rats, bilateral microinjection of nNOS antisense oligos (20 pmol in a 50 nl volume) into the NTS produced a significant increase in mean arterial blood pressure at 30–60 min after injection, compared with rats injected with nNOS sense or scrambled oligos. Immunohistochemical study demonstrated that nNOS immunoreactivity in the rat NTS was suppressed by nNOS antisense oligos. These results indicate that suppression of the nNOS gene using antisense in the NTS increases blood pressure. © 1999 Lippincott Williams & Wilkins, Inc.