Auditory and Vestibular SystemsInhibitory synaptic interactions between cochlear nuclei: Evidence from an in vitro whole brain studyBabalian, Alexander L.1,4; Ryugo, David K.2; Vischer, Mattheus W.3; Rouiller, Eric M.1Author Information 1Institute of Physiology and Program for Neuroscience, University of Fribourg, Rue du Musée 5, CH-1700 Fribourg, Switzerland 2Departments of Otolaryngology-Head and Neck Surgery and Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA 3ENT Clinic, University Hospital, Inselspital, Bern, Switzerland. 4Corresponding author: Alexander L. Babalian ACKNOWLEDGEMENTS: This work was supported by grants and financial contributions from Swiss National Science Foundation (No. 31-045731.95), CNRS (France), NIH/NIDCD (DC00232 and DC00979), Novartis Research Foundation and the Embassy of France in Switzerland. The experiments were conducted at the LNRS (Head: Dr P.-P. Vidal), CNRS (Paris). The authors would like to thank V. Moret and C. Roulin for their excellent technical assistance. Received 21 April 1999; accepted 27 April 1999 NeuroReport: June 23rd, 1999 - Volume 10 - Issue 9 - p 1913-1917 Buy Abstract USING guinea-pig isolated whole brain preparation in vitro, synaptic responses to electrical stimulation of auditory nerves were examined in intracellularly recorded and stained neurons of posteroventral and dorsal divisions of the cochlear nucleus. Stimulation of the contralateral auditory nerve evoked exclusively IPSPs in 70% of neurons, with amplitude of 2.3 ± 1.2 mV. Neurons of all major cell types were inhibited from the contralateral side. In the majority of responding cells (78%) IPSPs were induced at latencies of 3–9 ms suggesting di- and trisynaptic connections from contralateral auditory afferents or, respectively, mono- and disynaptic connections from the contralateral cochlear nucleus. Few cells responded with long-latency IPSPs (13.5–23 ms), indicating involvement of polysynaptic pathways. These data demonstrate the existence of functional, direct and indirect inhibitory connections between the cochlear nuclei. © 1999 Lippincott Williams & Wilkins, Inc.