Molecular NeuroscienceNeuronal loss up-regulates clusterin mRNA in living neurons and glial cells in the rat brainStrocchi, Paola1,2; Rauzi, Francesca1; Cevolani, Daniela1Author Information 1Department of Pharmacology, University of Bologna, Via Irnerio 48, I-40126 Bologna, Italy 2Corresponding Author: Paola Strocchi ACKNOWLEDGEMENTS: The authors wish to thank Professor Fiorenzo Stirpe for the generous gift of volkensin. We thank Dr Maria Marinelli for the excellent technical assistance and Dr Saori Takahama for the microphotographs. We are also most grateful to Professor Marina Bentivoglio for her suggestions in writing the manuscript. This study was supported by grants from the Ministero dell'Universita and the Italian Research Council MURST and CNR. Received 17 March 1999; accepted 13 April 1999 NeuroReport: June 3rd, 1999 - Volume 10 - Issue 8 - p 1788-1792 Buy Abstract THE aim of this study was to examine the time course and cellular localization of clusterin mRNA after neurodegeneration. Selective neuronal death was achieved in the rat inferior olivary complex after volkensin injection in the contralateral cerebellar cortex. Quantitative analysis of the in situ hybridization signal demonstrated over-expression of clusterin mRNA in living neurons at 6 days and outside the neuronal cell bodies at 10 days post-injection. We conclude that, in our experimental model, clusterin over-expression occurs as an early and transient neuronal and as a delayed glial response to selective neuronal death, supporting the view that clusterin may be involved in cytoprotection and tissue remodeling. © 1999 Lippincott Williams & Wilkins, Inc.