Molecular NeuroscienceMelatonin receptor subtype expression in human cerebellumAl-Ghoul, Walid M.1; Herman, Martin D.3; Dubocovich, Margarita L.1,2,4Author Information 1Department of Molecular Pharmacology and Biological Chemistry (S215), Northwestern University Medical School, 303 East Chicago Avenue, Chicago, IL 60611-3008, USA 2Northwestern Drug Discovery Program, Northwestern University Neuroscience Institute, Northwestern University Medical School, 303 East Chicago Avenue, Chicago, IL 60611-3008, USA 3Department of Neurosurgery, Lutheran General Hospital, Park Ridge, IL 60068, USA 4Corresponding Author: Margarita L. Dubocovich ACKNOWLEDGEMENTS: We would like to thank Dr Monica I. Masana and Dr Enrico Mugnaini for insightful comments on the manuscript, and Ms Leah Dickens for editorial help. This research was supported by USPHS grants MH 42922 to MLD and T32 ES 07124 to WMA. Received 16 September 1998; accepted 10 October 1998 NeuroReport: December 21st, 1998 - Volume 9 - Issue 18 - p 4063-4068 Buy Abstract WE report the distinct cellular distribution in the human cerebellar cortex of the mammalian mt1 (Mel1a) and MT2 (Mel1b) (1) melatonin receptor subtypes. Specific binding of the non-selective radioligand 2-[125I]iodomelatonin to the outer molecular layer was significantly higher than to the granule cell layer. Melatonin receptor subtype expression was assessed by in situ hybridization using selective and specific digoxigenin-labeled antisense oligonucleotide probes. This is the first demonstration of MT2 melatonin receptor mRNA expression in human cerebellar Bergmann glia and astrocytes. On the other hand, the mt1 melatonin receptor mRNA was expressed in both basket-stellate cells and granule cells. We conclude that mt1 and MT2 melatonin receptors are heterogeneously expressed in human cerebellar cortex. © 1998 Lippincott Williams & Wilkins, Inc.