Clinical NeuroscienceLiposomal transfection efficiency and toxicity on glioma cell lines: in vitro and in vitro studies*Bell, Helen1; Kimber, Wendy L.1; Li, Mingwei1; Whittle, Ian R.1,2Author Information 1Department of Clinical Neurosciences, Western General Hospital, Edinburgh, EH4 2XU, UK 2Corresponding Author: Ian R. Whittle ACKNOWLEDGEMENTS: W.K. was a Brain Tumour Research Fellow and M.L. was a British Council supported Sino-British Research Fellow. Professor D Porteous, MRC Human Genetics Unit, provided helpful advice. Received 21 October 1997; accepted 11 January 1998 NeuroReport: March 30th, 1998 - Volume 9 - Issue 5 - p 793-798 Buy Abstract TO evaluate the influence of cell type and cationic liposomal formulation on gene transfection efficiency three liposomes (lipofectin, lipofectamine and DOTAP) were used to transfect the human (A172 and MOG-GCCM) and rodent (C6 and A15A5) glioma cell lines with the Lac Z gene. Parallel studies evaluated in vitro cytotoxicity and the neuropathological changes following intracerebral injection. The transfection efficiency and cytotoxicity of the liposomes varied both quantitatively and qualitatively between the cell lines. There were no behavioural disturbances following intrastriatal or hippocampal injection and the neuropathological changes at the injection sites were focal and similar for all liposomes. The influence of glial cell lineage on both liposomal transfection facility and cytotoxicity may have important implications for in vivo gene transfection in the central nervous system. © Lippincott-Raven Publishers.