Membrane Biochemistry and BiophysicsIdentification of fucose α(1-2) galactose epitope-containing glycoproteins from rat hippocampusSmalla, Karl-Heinz1,2; Angenstein, Frank1,3; Richter, Karin1; Gundelfinger, Eckart D.1; Staak, Sabine1,4Author Information 1Federal Institute for Neurobiology, Department of Neurochemistry and Molecular Biology, D-39008 Magdeburg, POB 1860 2Institute of Pharmacology and Toxicology, Faculty of Medicine, Otto von Guericke University, Leipziger Strasse 44, 39120 Magdeburg, Germany 3Present address: Beckman Institute, NPA, University of Illinois, Urbana, IL 61801, USA 4Corresponding Author: Sabine Staak ACKNOWLEDGEMENTS: We thank Bert Seidel for help in monoclonal antibody production, Susanne tom Dieck for neuronal cell cultures, Angelika Reichel, Kathrin Zobel and Kathrin Baumann for expert technical assistance. We are grateful to Philip Beesley and Kristina Langnaese for gift of gp55/65 antibodies and helpful discussions. Supported by the Deutsche Forschungsgemeinschaft grant KR 1255/4-1 and the Fonds der Chemischen Industrie. Received 3 December 1997; accepted 13 January 1998 NeuroReport: March 30th, 1998 - Volume 9 - Issue 5 - p 813-817 Buy Abstract FUCOSYLATION of terminal galactose residues of brain glycoproteins in th eα(1–2) position has been shown to be crucial for neuronal plasticity, including phenomena such as long-term potentiation and long-term memory formation. We raised antibodies against the plasticity- relevant fu α(1-2)gal epitope and used them to determine the distribution of the epitope in adult rat hippocampus. To identify proteins bearing fu α(d-2)gal glycostructures antibodies against known synaptic fucoglycoproteins were used in combination with the fucα(1-2)gal antibodies. The NMDA receptor subunit NR1 and fractions of gp65 and cadherin were found to carry the epitope, while fucosylation of NCAM180 and NCAM140 obviously occurs via different linkages to the glycan chains. © Lippincott-Raven Publishers.