Regeneration and TransplantationLeukemia inhibitory factor maintains choline acetyltransferase expression in vivoCheema, S S.1,3; Arumugam, D1; Murray, S S.1; Bartlett, P F.2Author Information 1Department of Anatomy, Monash University, Clayton, Victoria 3168, Australia 2The Walter and Eliza Hall Institute of Medical Research, Post Office, Royal Melbourne Hospital, Victoria 3050, Australia 3Corresponding Author: S. S. Cheema ACKNOWLEDGEMENTS: We thank Ms Elizabeth Lopes for her expert technical assistance. This work was kindly supported by the Australian National Health and Medical Research Council, The AMRAD Corporation and The Co-operative Research Centre for Cellular Growth Factors. S.S.M. holds a Ph.D scholarship from the Bethlehem Griffiths Research Foundation of Australia. Website publication 3 February 1998 Received 12 November 1997; accepted 25 November 1997 NeuroReport: February 16, 1998 - Volume 9 - Issue 3 - p 363-366 Buy Abstract FOLLOWING axotomy most medial septal neurons in the adult rat brain have dramatically reduced numbers of choline acetyltransferase (ChAT) positive neurons. Since leukemia inhibitory factor (LIF) promotes cholinergic expression in several neuronal populations, the aim of this study was to determine if LIF would continue to support cholinergic expression in axotomized medial septal neurons. Mini-osmotic pumps were used to infuse saline or LIF into the lateral cerebral ventricle. Counts of ChAT and low-affinity nerve growth factor (p75NGFR) immunostained neurons indicated that LIF-treated animals retained ChAT expression in > 90% of axotomized neurons whereas in saline-infused animals this was < 30%. Also, LIF was equally effective in maintaining p75NGFR expression levels in axotomized medial septal neurons. © Lippincott-Raven Publishers.