Glial CellsInduction of manganese superoxide dismutase in BV-2 microglial cellsSugaya, Kiminobu1,2; Chouinard, Michael L.1; McKinney, Michael1Author Information 1Department of Pharmacology, Mayo Clinic Jacksonville, 4500 San Pablo Road, Jacksonville, FL 32224, USA 2Corresponding Author: Kiminobu Sugaya Received 6 August 1997; accepted 21 August 1997 NeuroReport: November 10th, 1997 - Volume 8 - Issue 16 - p 3547-3551 Buy Abstract THE regulation of the manganese-dependent superoxide dismutase (Mn-SOD) was studied in immortalized microglial cells (line BV-2). BV-2 cells, activated with lipopolysaccharide (LPS), exhibited an increase in MnSOD-like immunoreactivity, that was associated with an accumulation of nitrite in the culture medium and an increase in immunoreactivity for the inducible type of nitric oxide synthase (i-NOS). The i-NOS inhibitor L N6-(1-iminoethyl)-lysine (NIL, 600 μM) suppressed the nitrite accumulation and the increase in Mn-SOD-like immunoreactivity in activated cells without significant effect on the level of i-NOS-like immunoreactivity. The NO donor sodium nitroprusside dose-dependently increased Mn-SOD-like immunoreactivity in NILpretreated BV-2 cells. These results indicate that the induction of Mn-SOD in activated BV-2 cells is mediated in part by NO, or its metabolites. © Lippincott-Raven Publishers.