Chemical, Electrical and Magnetic SensesSubstrate-bound carbohydrates stimulate signal transduction and neurite outgrowth in an olfactory neuron cell linePuche, A C.2,4; Bartlett, P F.1; Key, B3Author Information 1Laboratory of Molecular Neurodevelopment, Department of Anatomy and Cell Biology, University of Melbourne, Parkville, 3052, Victoria, Australia 2Present address: Department of Anatomy and Neuroscience, School of Medicine, University of Maryland, 685 West Baltimore Street, Baltimore, MD 21201, USA 3Laboratory of Neuroimmunology, Walter and Eliza Hall Institute of Medical Research, Parkville, 3052, Victoria, Australia 4Corresponding Author: A. C. Puche ACKNOWLEDGEMENTS: This work was supported by grants from the National Health and Medical Research Council and from the Australian Research Council to B.K. A.C.P. was supported by an A.P.R.A. from the Australian Research Council. Website publication 24 September 1997 Received 24 July 1997; accepted 5 August 1997 NeuroReport: October 20th, 1997 - Volume 8 - Issue 15 - p 3183-3188 Buy Abstract SUBPOPULATIONS of olfactory receptor neurons, which are dispersed throughout the olfactory neuroepithelium, express specific cell surface carbohydrates and project to discrete regions of the olfactory bulb. Cell surface carbohydrates such as N-acetyl-lactosamine have been postulated to mediate sorting and selective fasciculation of discrete axon subpopulations during development of the olfactory pathway. Substrate-bound N-acetyl-lactosamine promotes neurite outgrowth by both clonal olfactory receptor neuron cell lines and olfactory receptor neurons in vitro, indicating that cell surface carbohydrates may be ligands for receptor-mediated stimulation of axon growth in vivo. In the present study, the role of transmembrane signaling in N-acetyl-lactosamine-stimulated neurite outgrowth was examined in the clonal olfactory neuron cell line 4.4.2. Substrate-bound N-acetyl-lactosamine stimulated neurite outgrowth which was specifically inhibited by antagonists to N- and L-type calcium channels and to tyrosine kinase phosphorylation. These results indicate that N-acetyl-lactosamine can evoke transmembrane receptor-mediated responses capable of influencing neurite outgrowth. © Lippincott-Raven Publishers.