Molecular NeuroscienceMultiple transcripts encode the 5-HT1F receptor in rodent brainGuptan, Preeta1; Dhingra, Anuradha1; Panicker, Mitradas M.1,2Author Information 1National Centre for Biological Sciences, TIFR Centre, PO Box 1234, I.I.Sc campus, Bangalore 560 012, India 2Corresponding Author: Mitradas M. Panicker ACKNOWLEDGEMENTS: The work was supported by grants from Tata Institute of Fundamental Research and the Department of Science and Technology, India. The authors would like to thank their colleagues at NCBS for constant encouragement and support. We also thank Dr Mahendra S. Rao (University of Utah) for helpful comments regarding the manuscript. Website publication 3 October 1997 Received 6 August 1997; accepted 20 August 1997 NeuroReport: October 20th, 1997 - Volume 8 - Issue 15 - p 3317-3321 Buy Abstract THE mouse serotonin 1F (5-HT1F) receptor is encoded by at least three transcripts in mouse brain. These transcripts are expressed predominantly in cortex and hippocampus. Similar transcripts are seen in Northern analysis of rat brain mRNA. 5' RACE showed a predominant transcription start site around 350 bp upstream of the translational start present in mouse cDNA. Our results suggest that the heterogeneity seen in transcript size is due to differences in the 3' untranslated region, which could play a critical role in mRNA targeting and localization. The mouse 5-HT1F genomic clone shows the coding region to be intronless and an intron splice junction is seen in the 5' untranslated region which is conserved in both rat and mouse. © Lippincott-Raven Publishers.