Molecular NeuroscienceExpression of peripherin, NADPH-diaphorase and NOS in the adult rat neocortexRhrich-Haddout, Fatiha1; Klosen, Paul2; Portier, Marie-Madeleine2; Horvat, Jean-Claude1,3Author Information 1Neurobiologie, URA CNRS 1448, Université René Descartes, 45 rue des Saints-Pères, 75270 Paris Cedex 06, France 2Cytosquelette et Développement, URA CNRS 2115, Faculté de Médecine Pitié-Salpêtrière, 105 bld de l'Hôpital, 75634 Paris Cedex 13, France 3Corresponding Author: Jean-Claude Horvat ACKNOWLEDGEMENTS: The study was supported by a grant from the 'Institut pour la Recherche sur la Moelle Epinièré (IRME). We thank Mrs L Klukvine for skillful technical assistance and Dr G Butler-Browne for critical revision of the English language. P.K. is the recipient of a long-term fellowship from the European Molecular Biology Organization. Website publication 29 September 1997 Received 15 July 1997; accepted 8 August 1997 NeuroReport: October 20th, 1997 - Volume 8 - Issue 15 - p 3313-3316 Buy Abstract PERIPHERIN is mainly expressed in peripheral neurones and in CNS neurones which extend axons into peripheral nerves. However, this intermediate filament protein has also been detected in a few other neurones entirely located within the CNS. The present study focuses on the adult rat neocortex. Peripherin immunoreactive (P+) neuronal somata and their neuritic extensions were observed in cortical layers II, III, V and VI, while a few P+ nerve fibres could be seen in layer I. All the P+ neurones could be selectively stained using reduced nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) histochemistry, a typical feature of aspiny neurones. Some of the P+ neurones could also be immunostained with an antibody raised against nitric oxide synthase (NOS). These results provide evidence that peripherin is present in a discrete population of aspiny interneurones of the adult rat neocortex. The functional significance of the co-expression of peripherin and NOS needs further investigation. © Lippincott-Raven Publishers.