Regeneration and Transplantationα1-tubulin mRNA level is increased during neurite outgrowth of NG 108-15 cells but not during neurite outgrowth inhibition by CNS myelinKnoops, Bernard1,3; Octave, Jean-Noël2Author Information 1Catholic University of Louvain, Laboratory of Neurochemistry, Clos Chapelle-aux-Champs, 30–31, 1200 Brussels, Belgium 2Present address: Catholic University of Louvain, Unit of Industrial Toxicology and Occupational Medicine, Clos Chapelle-aux-Champs, 30–54, 1200 Brussels, Belgium 3Corresponding Author and Address: Bernard Knoops, Catholic University of Louvain, Unit of Industrial Toxicology and Occupational Medicine, Clos Chapelle-aux-Champs, 30-54, 1200 Brussels, Belgium ACKNOWLEDGEMENTS: We would like to thank Mr Ferdinand Desneux for his excellent photographic assistance. J.N.O. is Senior Research Associate of the National Research Funds of Belgium. This work was supported by grant No. 9.44545.95 F from the National Research Funds of Belgium and a grant from the Belgian Queen Elisabeth Medical Foundation. Received 16 October 1996; accepted 14 November 1996 NeuroReport: February 10th, 1997 - Volume 8 - Issue 3 - p 795-798 Buy Abstract 1-TUBULIN is an isotype of α-tubulin, and its mRNA is expressed in the rodent nervous system. A high level of α1-tubulin mRNA in neurones is associated with axonal outgrowth during development as well as with axonal regeneration after axotomy in adult animals. We quantitated α1-tubulin mRNA levels in motor neurone-like NG 108-15 cells using Northern blots in order to determine whether the expression of this neurite outgrowth-associated gene is regulated in NG 108-15 cells during neurite extension and during inhibition of this process by CNS myelin. Here we report that during the acute phase of neurite outgrowth, α1-tubulin mRNA level increases in NG 108-15, a maximal induction of 1.7-fold over the initial level occurring 24 h after neurite outgrowth onset. By contrast, when these cells are plated on CNS myelin α1-tubulin mRNA levels show no such increase. These findings indicate that an increase of the α1-tubulin mRNA level is associated with neurite outgrowth of NG 108-15 cells. More interestingly, this study also demonstrates that the inhibition of neurite outgrowth by CNS myelin may affect the expression of a gene encoding a protein involved in neurite extension. © Lippincott-Raven Publishers.