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Selective expression of c-mas proto-oncogene in rat cerebral endothelial cells

Kumar, Murukambat1; Grammas, Paula1,3; Giacomelli, Filiberto2; Wiener, Joseph2

Molecular Neuroscience

NORTHERN blot analysis of cultured endothelial cells derived from rat cerebral resistance vessels demonstrated the presence of c-mas mRNA. This is the first description of mas expression in non-neuronal cells. c-mas message was not detectable in cultured endothelial cells derived from other vascular beds, including rat aorta and mesenteric resistance vessels. Since c-mas has been purported to regulate the proliferation response to angiotensin, the growth properties of all three endothelial cell cultures were examined. The data indicated no differences in either basal or angiotensin-stimulated cell growth among brain, mesenteric or aortic-derived endothelial cells. These data suggest that c-mas is selectively expressed in brain endothelial cells and that this proto-oncogene does not regulate cell proliferation in this cell type.

1Department of Pathology, University of Oklahoma Health Sciences Center, P.O. Box 26901, Oklahoma City, OK 73104

2Department of Pathology, Wayne State University, Detroit MI 48201, USA

3Corresponding Author: Paula Grammas

ACKNOWLEDGEMENTS: The authors thank Toya Botchlet for technical assistance. This work was supported in part by NIH grants NS30457 and HL 23603.

Received 16 August 1996; accepted 30 August 1996

© Lippincott-Raven Publishers.