Neuroimmunology: PDF OnlyIL-1β, IL-1 receptor type I and iNOS gene expression in rat brain vasculature and perivascular areasWong, Ma-Li; Bongiorno, Peter B.; Al-Shekhlee, Amer; Esposito, Anna; Khatri, Pooja; Licinio, JulioAuthor Information Clinical Neuroendocrinology Branch, Intramural Research Program, NIMH, NIH, Bldg. 10, Rm. 3S231, 10 Center Dr MSC 1284, Bethesda, MD 20892–1284, USA NeuroReport: November 4, 1996 - Volume 7 - Issue 15 - p 2445-2448 Buy Abstract THE mechanism by which IL-1β exerts its actions in the brain during systemic inflammation is not fully understood, as neither IL-1 receptor gene expression nor IL-1 binding have been identified in significant levels in key areas that respond to IL-1β. Having hypothesized that perivascular nitric oxide (NO) might modulate the effects of systemic IL-1β in the brain, we studied the expression of the genes encoding for IL-1β, the signal-transducing IL-1 receptor type I (IL-1RI) and inducible NO synthase (iNOS) constitutively and during systemic inflammation in vascular and perivascular regions of the rat brain. Our results show that IL-1RI is constitutively expressed at the interface of the vascular wall and perivascular glia. During systemic inflammation there is induction of IL-1 (3 gene expression in the vascular wall, accompanied by perivascular induction of iNOS mRNA. We conclude that during systemic inflammation vascular IL-1 (3, binding to vascular and perivascular IL-1RI receptors, may induce perivascular iNOS gene expression, leading to the production of NO and modulation of the effects of IL-1 (3 in the brain. We propose that the vascular and perivascular induction of iNOS mRNA by IL-1β might represent a mechanism for the modulation of the central nervous system effects of peripheral inflammatory mediators. © Lippincott-Raven Publishers.