Neuropharmacology and Neurotoxiology: PDF OnlyInhibition of nitric oxide synthase protects against hypoxic neuronal injuryWallis, Roi Ann; Panizzon, Kimberly; Wasterlain, Claude G.Author Information Department of Neurology, UCLA School of Medicine, Sepulveda VAMC (111-N1), 16111 Plummer St., Sepulveda, CA 91343 USA NeuroReport: July 1992 - Volume 3 - Issue 7 - p 645-648 Buy Abstract We investigated the action of nitric oxide in hypoxic neuronal injury, using the hippocampal slice. Inhibition of nitric oxide synthase with the competitive inhibitor, NG-monomethyl-L-arginine, provided significant protection against hypoxia for population spike, EPSP and fiber volley responses, with an EC50 of 30 $mUM for protection of antidromic population spike amplitude. Confirming a stereo-specific site of action, this protection was reversed by the addition of L-arginine, but not D-arginine. These results indicate that nitric oxide generation may mediate acute CA1 neuronal injury during hypoxia, and that inhibition of nitric oxide synthase may be a useful neuroprotective strategy. © Lippincott-Raven Publishers.