Regeneration and Transplantation: PDF OnlyHepatocytes enhance neurite regeneration and survival from transected nerve terminalsHorie, Hidenori; Fukuda, Naoto; Bando, YokoAuthor Information Department of Physiology, School of Medicine, Yokohama City University, 3–9 Fukuura, Kanazawaku, Yokohama, 236 Japan NeuroReport: September 1991 - Volume 2 - Issue 9 - p 521-524 Buy Abstract THE culture of hepatocytes dissociated from adult mice by the collagenase perfusion method enables neurons or neuronal tissues to be cultured with hepatocytes or in hepatocyte-conditioned media. Co-culture with hepatocytes or hepatocyte-conditioned media enhances neurite regeneration and their survival from nerve-transected terminals of dorsal root ganglia with nerve fibers dissected from adult and aged mice. Hepatocytes secrete a factor which enhances not only neurite regeneration but also neurite survival. Activities of other known neurotrophic factors were not as crucial as those of the hepatocyte-conditioned medium, suggesting that this factor may differ from other trophic ones. © Lippincott-Raven Publishers.